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DTSTART;TZID=Africa/Casablanca:20221009T093000
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DTSTAMP:20260404T205407
CREATED:20220501T070132Z
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UID:1331-1665307800-1665342000@www2.mserm-congress.org
SUMMARY:The 6th MSERM WORKSHOP - Clinical and Biological Aspects of Performing an Efficient Oocyte Pick Up "OPU"
DESCRIPTION:Virtual Event is on Sunday 9 October 2022 \nUltrasound-guided oocyte pick-up (OPU) is a well-established procedure during which ovarian follicles are aspirated with a needle connected to a suction pump. The suction pump creates negative pressure for aspirating the ovarian follicles. \n An efficient aspiration pressure will maximize the oocyte recovery rate and reduce the risks of drastic temperature changes and mechanical stress to the oocyte. Therefore\, it is important to notice that a higher aspiration pressure is not necessarily associated with any damage to the oocytes\, especially if the the flow rate is stable and has been set according to the needle size and the manufacturer’s recommendations. \nContinuous vacuum suction should be used just before penetrating the follicle. The positive pressure inside the follicle is dependent on the size\, shape and the intraovarian position of the follicle. As the follicle matures it produces more follicular fluid. This increases the intrafollicular pressure causing the bulging of the external surface\, especially when the follicle has reached maturity. The pressure of the fluid in the follicle at the moment of needle penetration might be significantly higher than the normal follicular pressure. As the needle tip is forced into the wall\, the deformation of the follicular surface will cause the pressure to rise even further.  \nA stable flow rate should be maintained during the entire procedure\, because changes can induce turbulence. If turbulent flow is present\, the oocyte may be tossed around\, which could result in the cumulus cells being stripped off or the cracking of the zona pellucida. An intact cumulus layer is an important factor contributing to the resistance of oocytes to mechanical damage. Provided the aspiration pressure is maintained constant\, the fluid velocity is the same throughout the needle and tubing line\, if their diameters are also constant. Any diameter changes in the aspiration system (at the needle or in tubing line) will lead to sudden changes in flow rate\, creating unwanted pressure changes and the presence of turbulent flow leading to mechanical stress of the oocyte. \nAs the follicular fluid volume is aspirated\, the follicle collapses which should be continuously monitored by the ultrasound in order not to lose any oocytes. If complete follicle emptying cannot be verified\, the oocyte can remain trapped inside the cavity). At this point\, the needle should be gently withdrawn without applying any negative aspiration pressure to avoid a sudden forward flow of the follicular fluid towards the collection tube. \nIn conclusion\, some of the most important practical considerations for an efficient and successful egg retrieval are: \nSetting the right aspiration pressure depending on the the thickness of the utilized needle (gauge).\nApplying continuous vacuum suction before penetrating the follicle.\nMaintaining a stable pressure throughout the entire procedure\nAvoiding applying negative aspiration pressure when withdrawing the needle from the follicle. \nBibliography: \nAwonuga A\, Waterstone J\, Oyesanya O\, Curson R\, Nargund G\, Parsons J. A prospective randomized study comparing needles of different diameters for transvaginal ultrasound-directed follicle aspiration. Fertil Steril 1996;65:109–113. \nAziz N\, Biljan MM\, Taylor CT\, Manasse PR\, Kingsland CR. Effect of aspirating needle calibre on outcome of in-vitro fertilization. Hum Reprod 1993;8:1098–1000. \nThe ESHRE Working Group on Ultrasound in ART\, D’Angelo A\, Panayotidis C\, Amso N\, Marci R\, Matorras R\, Onofriescu M\, Berkiz Turp A\, Vandekerckhove F\, Veleva Z\, Vermeulen N\, Vlaisavljevic\, Recommendations for good practice in ultrasound: oocyte pick up Human Reproduction Open 2019;4:1–25. \nIduna Antigoni Buisman ET\, de Bruin JP\, Maria Braat DD\, van der Steeg JW. Effect of needle diameter on pain during oocyte retrieval-a randomized controlled trial. Fertil Steril. 2021;115:683-691. \nKumaran A\, Narayan PK\, Pai PJ\, Ramachandran A\, Mathews B\, Adiga SK. Oocyte retrieval at 140mmHg negative aspiration pressure: a promising alternative to flushing and aspiration in assisted reproduction in women with low ovarian reserve. J Hum Reprod Sci 2015;8:98–102. \nKushnir VA\, Kim A\, Gleicher N\, Barad DH. A pilot trial of large versus small diameter needles for oocyte retrieval. Reprod Biol Endocrinol. 2013;11:22. \nNakagawa K\, Nishi Y\, Kaneyama M\, Sugiyama R\, Motoyama H\, Sugiyama R.J. The Effect of a Newly Designed Needle on the Pain and Bleeding of Patients during Oocyte Retrieval of a Single Follicle. Reprod Infertil. 2015;16:207-11. \nRose BI. Approaches to oocyte retrieval for advanced reproductive technology cycles planning to utilize in vitro maturation: a review of the many choices to be made. J Assist Reprod Genet. 2014;31:1409-19.
URL:https://www2.mserm-congress.org/event/the-6th-mserm-workshop-clinical-and-biological-aspects-of-performing-an-efficient-oocyte-pick-up-opu/
CATEGORIES:Workshop
ATTACH;FMTTYPE=image/png:https://www2.mserm-congress.org/wp-content/uploads/2022/05/6th-MSERM-WORKSHOP.png
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20220923
DTEND;VALUE=DATE:20220924
DTSTAMP:20260404T205407
CREATED:20220319T114032Z
LAST-MODIFIED:20220319T124346Z
UID:1292-1663891200-1663977599@www2.mserm-congress.org
SUMMARY:MSERM workshop – Virtual event – Revisiting standards in the IVF laboratory: Improvement outcomes with precision treatment
DESCRIPTION:
URL:https://www2.mserm-congress.org/event/mserm-workshop-virtual-event-revisiting-standards-in-the-ivf-laboratory-improvement-outcomes-with-precision-treatment/
CATEGORIES:Workshop
ORGANIZER;CN="MSERM":MAILTO:info@mserm.org
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Paris:20220422T094000
DTEND;TZID=Europe/Paris:20220422T170000
DTSTAMP:20260404T205407
CREATED:20220319T112500Z
LAST-MODIFIED:20220319T120033Z
UID:1286-1650620400-1650646800@www2.mserm-congress.org
SUMMARY:MSERM workshop – Virtual event – Ovarian Endometriosis
DESCRIPTION:
URL:https://www2.mserm-congress.org/event/mserm-workshop-virtual-event-ovarian-endometriosis/
CATEGORIES:Workshop
ORGANIZER;CN="MSERM":MAILTO:info@mserm.org
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